Cell Signaling
N-Cadherin (D4R1H) XP® Rabbit mAb #13116
D4R1H
N/A
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Very good antibody tested in WB. Used on PVDF membrane, primary diluted 1:1000 in 5% BSA and secondary used 1:5000 for 2 h. Bands are clean and predicted molecular weight is accurate. Recommended.
Western Blot
A549
1:1000 overnight
5% BSA in TBST 1x
1:5000 2 hours
Very good antibody tested in WB. I used this antibody in A549 lysate evaluating epithelial mesenchymal transition and used just 10 micrograms of lysate. Used on PVDF membrane, primary diluted 1:1000 in 5% BSA and secondary used 1:5000 for 2 h. Bands are clean and predicted molecular weight is accurate. Recommended.
Very clean band.
Great, I will buy again.