Great CD31 Antibody for Flow Cytometry

May 17, 2022

Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Fisher Scientific

Product Name:

CD31 Rat anti-Mouse, PE

Catalog Number:

BDB561073

Clone Number:

MEC 13.3

Endothelial cells were isolated from mouse brain tissue for in vitro culturing. CD31 Rat anti-mouse PE antibody was used to assess enrichment of endothelial cells from mouse brain tissue by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse brain tissue homogenate

Primary Incubation

1:100, 30 minutes on ice

Blocking Agent

TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytoflex S Flow Cytometer (Beckman Coulter)

Results Summary

CD31+ endothelial cells were brightly labeled and clearly detectable by flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Inexpensive compared to other CD31 antibodies.

The Bad

None to report.

The Bottom Line

This is the least expensive conjugated CD31 antibody we could find and it works very well to identify endothelial cells by flow cytometry.

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