Great Fibronectin Antibody

May 16, 2022

BlueskyReddit
Translational Glycomics
Versiti Blood Research Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma Aldrich

Product Name:

Anti-Cellular Fibronectin antibody, Mouse monoclonal clone FN-3E2, purified from hybridoma cell culture

Catalog Number:

SAB4200880

Clone Number:

FN-3E2

Anti cellular fibronectin antibody was used to compare fibronectin levels in WT and PACSIN2 knockout mouse plasma samples in Western blots using reduced and non-reduced conditions. Knockout mice showed bands in the western blots in non-reduced conditions.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse plasma

Primary Incubation

1:2000, 2 hrs, 4*C

Blocking Agent

BSA

Secondary Incubation

1:5000, 1 hr, 4*C

Tertiary Incubation

None

Detection

Chemiluminescence by Image quant

Results Summary

Anti cellular fibronectin antibody was used to compare fibronectin levels in WT and PACSIN2 knockout mouse plasma samples in Western blots using reduced and non-reduced conditions. Knockout mice showed bands in the western blots in non-reduced conditions.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and good signal

The Bad

None

The Bottom Line

Antibody has good signal. Would recommend it and buy again.

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