CD31+ Cell Enrichment from Mouse Cortex

University of Illinois at Chicago
Anatomy and Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD31 MicroBeads, mouse

Catalog Number:

130-097-418

CD31 MicroBeads were used to isolate CD31+ endothelial cells from mouse cortical tissue for transcriptome analysis.

Experimental Design and Results Summary

Application

Single cell RNA sequencing

Starting Material

Mouse cortex

Tips

Columns require vigorous washing for optimal enrichment.

Results Summary

CD31+ endothelial cells were enriched 3-fold from 15% to 60%.

DOI or PMID #

N/A

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Summary

The Good

Fast protocol

The Bad

Specific columns and magnet rack required to use.

The Bottom Line

While CD31+ endothelial cells were greatly enriched from the starting culture, a large amount of non-CD31+ cells remained. Further optimization or steps outside the manufacturer provided protocol will be needed to achieve better enrichment.

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