Millipore Sigma
Duolink® In Situ Detection Reagents Red
DUO92008
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I used this with HEK293 cells to detect protein protein interactions. Kit is easy to use and the company provides a lot of troubleshooting support. Interestingly, the Reagents Red can be detected using Cy5 fluorescence channel. Using Texas Red does give a better signal, it can be done.
PLA
HEK 293 cells
Coat the slides to aid in cell adherence due to washing steps.
Was able to detect novel protein interaction.
N/A
Works great!
No cons.
Highly recommend.