Cell Signaling Technology
Ras (G12V Mutant Specific) (D2H12) Rabbit mAb
14412
N/A
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This antibody is specific for the detection of mutant RAS in the codon G12V. It cannot be used for other RAS mutant.
Western Blot
Mammary epithelial cell line, MCF10A
1:1000. Overnight. Cold room.
5% Milk in PBS
1:7000. 1 hour at room temperature.
Bioluminescence (HRP-conjugated)
Mutant RASg12v was overexpressed through an inducibile doxycycline-dependent system. The ovexpression was strongly detected by this antibody after 24h of induction.
It gives a sharp and specific band.
Quite costly although very good antibody.
Very recommended antibody!