Antibody Resulted in Non-Specific Bands

May 11, 2022

BlueskyReddit
Neurosurgery
Penn State College of Medicine
Graduate Student

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Company:

Cell Signaling

Product Name:

TNF-alpha antibody

Catalog Number:

3707S

Clone Number:

N/A

Used this antibody on human astrocytes at 1:1000 dilution. Only got bands at 50 kD which is MUCH higher than TNF-a, unless it dimerized despite denaturing.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human astrocytes

Primary Incubation

1:1000 overnight BSA

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Tried to demonstrate astrocyte activation via amyloid beta by measuring TNF-a levels.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Not much.

The Bad

Bands not in correct place.

The Bottom Line

Would not recommend.

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