Great Beta-Actin Antibody for WB

May 10, 2022

BlueskyReddit
Nanomedicine
Lazar Research Laboratories
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell signaling

Product Name:

β-Actin (8H10D10) Mouse mAb

Catalog Number:

3700

Clone Number:

N/A

I used bactin as housekeeping gene several times. I tested on different cell lines and I have never had problems. I tested on fibroblast and epithelial cells stimulated with tgf-b up to 24 hours and it worked perfectly.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast, Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I used GAPDH as housekeeping gene several times. I tested on different cell lines and I have never had problems. I tested on fibroblast and epithelial cells stimulated with tgf-b up to 24 hours and it worked perfectly. The cells have grown up in starvation medium.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Antibody has great signal, highly recommended.

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