Good N-Cadherin Antibody for WB

May 09, 2022

BlueskyReddit
Nanomedicine
Lazar Research Laboratories
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

N-Cadherin

Catalog Number:

4061S

Clone Number:

N/A

I am very satisfied with this antibody. I tested it on endothelial cells (A549) stimulated with TGF-b for 24 hours in starvation medium (F-12k). The bands have been very well detected. My goal was to evaluate the epithelial mesenchymal transition.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Clear bands. I have never had problems with this antibody. I wanted to evaluate the epithelial mesenchymal transition on A549 stimulated with TGF-b for 24 hours with starvation medium.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

I'm really satisfied and I recommend to buy it.

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