Amazing Antibody for WB and PLA

May 06, 2022

Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz

Product Name:

TfR Antibody

Catalog Number:

SC-65882

Clone Number:

N/A

Used for proximity ligation assay and works great on HEK 293 cells at 1:200 dilution. Works for WB using human iPSC-derived Ecs and mouse brain tissue at 1:1000.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK 293 cells

Primary Incubation

1:250

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Used to show interaction with Tf as a positive control. Also used to detect TfR levels in mouse tissue and human cells WB at dilution of 1:250.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great for all applications.

The Bad

None

The Bottom Line

Always recommend, it is my go-to.

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