Good Antibody For WB and PLA

May 04, 2022

Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

TfR Antibody

Catalog Number:

13208S

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Used for proximity ligation assay and works great on HEK 293 cells at 1:200 dilution. Works for western blot using human iPSC-derived Ecs and HEK 293 at 1:1000.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK 293 cells

Primary Incubation

1:1000 overnight BSA

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Used to determine TfR levels in treated cells. Image shows ladder on either side of sample.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well.

The Bad

Few non-specific bands.

The Bottom Line

Would recommend.

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