Wrong Band from HRP Antibody

May 03, 2022

Medicine
University of California San Francisco
Postdoctoral Researcher

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Quality of Results

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Company:

BioLegend

Product Name:

HRP anti-TFEB Antibody

Catalog Number:

852003

Clone Number:

A17106C

Info:Supplier Page View Product Specs

Browse Similar Products: HRP Antibodies

Use this for IP-western blot for my mouse sample, but shows clear band with wrong molecular weight when comparing to the other two validated anti-TFEB produced be Bethyl and Proteintech.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse macrophage

Primary Incubation

1:10000, RT, 2h

Blocking Agent

5% non-fat milk/TBST

Secondary Incubation

None

Tertiary Incubation

None

Detection

ECL

Results Summary

This antibody detects a wrong band when comparing to the other two validated anti-TFEB from Bethyl and Proteintech.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

None

The Bad

Wrong band

The Bottom Line

Not recommend

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