Great Clec4f Antibody for IF

May 02, 2022

Institute of Microbiology, Infectious Diseases and Immunology
Charité – Universitätsmedizin Berlin
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Clec4f

Catalog Number:

156804

Clone Number:

3E3F9

Info:Supplier Page View Product Specs

Browse Similar Products: CLEC4F Antibodies

We are interested to study tissue-resident macrophages in various. For this, we wanted to identify the liver-resident Kupffer cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed 18 μm cryosections of mouse liver from a 10 week old C57BL/6J mouse

Primary Incubation

1:100 overnight at room temperature

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Excellent straining of Kupffer cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very nice staining with no background.

The Bad

None

The Bottom Line

Great antibody for staining Kupffer cells.

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