ß3 Tubulin for IF

Institute of Microbiology, Infectious Diseases and Immunology
Charité – Universitätsmedizin Berlin
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

β3 Tubulin

Catalog Number:

801207

Clone Number:

TUJ1

We are interested in studying tissue-resident macrophages in the kidney and their interactions with other stromal cells. For this, we wanted to identify neurons in the kidney.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed 14 μm cryosections of mouse kidney from a 10 week old C57BL/6J mouse

Primary Incubation

1:100 overnight at room temperature

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Excellent staining of neurons, with no background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining of neurons.

The Bad

None

The Bottom Line

Highly recommended.

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