Invitrogen
Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit
C10456
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Good alternative to radioactive ways of measuring protein synthesis. Straightforward OPP puro treatment and then subsequent fixation and click reaction for use with flow cytometry in this case.
Flow cytometry
Immortalized cell lines
Make sure to have replicates.
Helped us show differences in protein synthesis rate between different conditions.
N/A
Easy to use
Not sure about how variable results are.
Overall a good assay. Make sure to have some negative controls for protein synthesis (i.e. cyclohexamide treatment).