Time Course Effect of Chemoattractant on GPR75 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Invitrogen

Product Name:

GPR75 Polyclonal Antibody

Catalog Number:

PA5-97804

Clone Number:

N/A

Info:Supplier Page View Product Specs

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GPR75 is a G protein coupled receptor 75 that is encoded by the GPR75 gene. GPRs are present on the cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand. Here we checked the time course effect of GPR75 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:500 in 1x TBST prepared in skim milk

Blocking Agent

5% TBST prepared in skim milk

Secondary Incubation

1:4000 in 1x TBST prepared in skim milk

Tertiary Incubation

None

Detection

ECL

Results Summary

We found no change in GPR75 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This is a high concentration antibody and works great for EMSA.

The Bad

None

The Bottom Line

Works very well for western blot. Highly recommended.

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