Good, Usable Anti-ß-Catenin Antibody

April 28, 2022

BlueskyReddit
Department of Pediatrics
Children's mercy hospital
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified Mouse Anti-β-Catenin

Catalog Number:

610153

Clone Number:

N/A

We used it to detect Beta-Catenin monoclonal antibody specifically binds to Beta-Catenin. β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human cancer tissue

Primary Incubation

1:250 dilution overnight incubation

Blocking Agent

5% GS

Secondary Incubation

Incubate secondary antibody in 5% gs in PBS for one hour with an orbital shaker at room temperature.

Tertiary Incubation

N/A

Detection

Confocal microscope.

Results Summary

Link between β-Catenin and colon cancer has been described by β-Catenin staining.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal.

The Bad

N/A

The Bottom Line

Can be good used.

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