Great Alpha Smooth Muscle Actin Antibody

Nanomedicine
HMRI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

Catalog Number:

ab7817

Clone Number:

N/A

I use this antibody every time as a housekeeping gene and to normalize my Western Blot results in A549 and MRC5 cells. I use it at a dilution of 1:1000, followed by a secondary antibody 1:5000 for 2 hours but it can even be diluted further. Bands look very good. Higly recommended.

Experimental Design and Results Summary

Applications

Western Blot

Sample

A549, MRC5 cells

Primary Incubation

1:1000 overnight

Blocking Agent

5% BSA in TBST 1x

Secondary Incubation

1:5000 2 hours

Tertiary Incubation

N/A

Detection

Western Blot

Results Summary

Very good quality. Recommended.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specificity

The Bad

Nothing to complain about.

The Bottom Line

Very good quality. Recommended.

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