Abcam
Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
ab7817
N/A
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Browse Similar Products: Alpha Smooth Muscle Actin Antibodies
I use this antibody every time as a housekeeping gene and to normalize my Western Blot results in A549 and MRC5 cells. I use it at a dilution of 1:1000, followed by a secondary antibody 1:5000 for 2 hours but it can even be diluted further. Bands look very good. Higly recommended.
Western Blot
A549, MRC5 cells
1:1000 overnight
5% BSA in TBST 1x
1:5000 2 hours
Very good quality. Recommended.
Specificity
Nothing to complain about.