Cell Signaling
β-Actin Mouse mAb
8H10D10
N/A
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I used this antibody with the aim to evaluate mitochondria biogenesis pathway in A549 and MRC5 cells. After applying the conditions suggested by the vendor, WB bands were very clean and easy to quantify. Molecular weight described by the vendor is accurate. Highly recommended.
Western Blot
A549, MRC5 cells
1:1000 overnight
5% BSA in TBST 1x
1:5000 1 hours
NA
Excellent even when reused many times.
Very clean band.
Nothing to complain about.
Excellent overall.