Reliable Western Blot Antibody for HMGCS2

April 22, 2022

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-HMGCS2 antibody

Catalog Number:

ab137043

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: HMGCS2 Antibodies

We are investigating insulin-dependent and insulin-independent signaling pathways under the context of obesity. This antibody from Abcam showed good signal across all samples.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse liver lysate

Primary Incubation

1:1000 in 3% BSA in TBST buffer

Blocking Agent

5% non fat milk in TBST buffer

Secondary Incubation

1:10,000

Tertiary Incubation

NA

Detection

HRP development

Results Summary

Great detection of hmgcs2 in the liver lysate. As expected, Hmgcs2 knockdown mouse samples have less Hmgcs2 expression in the liver.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal across all samples.

The Bad

N/A

The Bottom Line

Reliable antibody

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