Good Anti-Mouse CD18

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD18 Antibody

Catalog Number:

101407

Clone Number:

M18/2

Info:Supplier Page View Product Specs

Browse Similar Products: CD18 Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we were analysing surface molecules expressed by PCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Surface staining with CD18 PE (1/100), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometer

Results Summary

PCs express intermediate amounts of CD18.

DOI or PMID #

https://doi.org/10.3389/fimmu.2022.873217

Additional Notes

N/A

Image Gallery

Summary

The Good

It works well.

The Bad

It is not that bright.

The Bottom Line

It can be used easily.

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