Good Option To Stain Mouse CD326

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM) Antibody

Catalog Number:

118231

Clone Number:

G8.8

Info:Supplier Page View Product Specs

Browse Similar Products: CD326 Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at finding surface markers of PCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Surface staining with CD326 BV510 (1/200), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

PCs express CD326.

DOI or PMID #

https://doi.org/10.3389/fimmu.2022.873217

Additional Notes

N/A

Image Gallery

Summary

The Good

It stains pretty well.

The Bad

It is not very bright.

The Bottom Line

It works!

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