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PE/Dazzle™ 594 anti-mouse CD39 Antibody
143812
Duha59
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at finding surface markers of PCs.
Flow Cytometry
Mouse spleen
Surface staining with CD39PEDazzle594 (1/400), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We could stain PCs (CD138hi) with anti-CD39.
https://doi.org/10.3389/fimmu.2022.873217
It is bright.
It works very well!