Miltenyi Biotec
CD49d Antibody, anti-mouse, REAfinity
130-111-979
REA807
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. In this set of experiments, we were looking for PC surface markers.
Flow Cytometry
Mouse spleen
Surface staining with CD49d PE-Vio770 (1/200), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
CD49d is expressed at the surface of PC (CD138hi cells).
https://doi.org/10.3389/fimmu.2022.873217
It is bright
There is some background staining
Good option to stain CD49d