Very Good Anti-Mouse CD49d

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD49d Antibody, anti-mouse, REAfinity

Catalog Number:

130-111-979

Clone Number:

REA807

Info:Supplier Page View Product Specs

Browse Similar Products: CD49d Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. In this set of experiments, we were looking for PC surface markers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Surface staining with CD49d PE-Vio770 (1/200), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

CD49d is expressed at the surface of PC (CD138hi cells).

DOI or PMID #

https://doi.org/10.3389/fimmu.2022.873217

Additional Notes

N/A

Image Gallery

Summary

The Good

It is bright

The Bad

There is some background staining

The Bottom Line

Good option to stain CD49d

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