Thermo Fisher Scientific
CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), eBioscience™
14-0711-82
N/A
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We used the antibody to stain brain mouse samples through IHC (IF). Different optimizations were performed, including the addition of a permeabilization step with Triton X-100 (0.1; 0.25% or without Triton), different incubation times, and concentrations of primary antibody (1:50; 1:100; 1:250). It never worked.
Immunohistochemistry
Mouse brain
1:50
0.1% BSA + Normal Goat serum (1:60)
1:1000
AlexaFluor 594 goat anti-rat
Fluorescence
The antibody doesn't stain for mouse brain samples. The small dots correspond to lipofuscin accumulation.
Does not work to IHC
It may work for other applications