Cell Signaling
Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425)
8828
N/A
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Cells were stimulated with TGF-b in 0.5% FBS for 24 hours. Our research is focused on the SMAD pathway that regulates a diverse set of biological processes, including proliferation, differentiation and apoptosis, in many different organ systems. TGF-ß ligands that activate the SMAD-2/3 intracellular pathway have been heavily implicated in fibrosis.
Western Blot
Fibroblast
1:1000, 4 °C, overnight
BSA 5% TBST
1:10000
Chemiluminescence
Data are not always clear; but antibody works sometimes
Band detected (not always)
Sometimes I got only one band instead of 2
Expensive for the quality