Bright CD29 Staining on Human Platelets

April 18, 2022

Hem/Onc
Boston Children's Hospital
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

APC/Cyanine7 anti-human CD29 Antibody

Catalog Number:

303014

Clone Number:

TS2/16

Info:Supplier Page View Product Specs

Browse Similar Products: CD29 Antibodies

Platelet surface CD29 is a β1 integrin that associates with integrin α-chains and mediates plateletadhesion. APC/Cyanine7 anti-human CD29 Antibody provides bright staining on human platelets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood collected in sodium citrate

Primary Incubation

30 min at room temperature

Blocking Agent

Staining buffer contains 0.5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing APC/Cyanine7 anti-human CD29 Antibody. Lyse/fix buffer was added, and samples were acquired by flow cytometry. APC/Cyanine7 anti-human CD29 Antibody allowed the clear resolution of platelets from background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining of platelet CD29

The Bad

N/A

The Bottom Line

APC/Cyanine7 anti-human CD29 Antibody allows bright staining of CD29 on the platelet surface.

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