Bright CD31 Antibody Staining

Hem/Onc
Boston Children's Hospital
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

Brilliant Violet 785 anti-human CD31 Antibody

Catalog Number:

303148

Clone Number:

WM59

Info:Supplier Page View Product Specs

Browse Similar Products: CD31 Antibodies

CD31 is a transmembrane protein that inhibits hemi-ITAM and ITAM-mediated platelet signaling. Brilliant Violet 785 anti-human CD31 Antibody provides bright staining on human platelets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood collected in sodium citrate

Primary Incubation

30 min at room temperature

Blocking Agent

Staining buffer contains 0.5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing Brilliant Violet 785 anti-human CD31 Antibody. Lyse/fix buffer was added, and samples were acquired by flow cytometry. Brilliant Violet 785 anti-human CD31 Antibody allowed good staining of CD31 on the surface of human platelets.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright signal allows good CD31 staining.

The Bad

N/A

The Bottom Line

Brilliant Violet 785 anti-human CD31 Antibody allows the clear detection of CD31 on human platelets.

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