Great CD4 Antibody for Flow Cytometry

April 18, 2022

BlueskyReddit
Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

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Company:

BioLegend

Product Name:

APC/Fire 750 anti-mouse CD4 Antibody

Catalog Number:

100460

Clone Number:

GK1.5

Info:Supplier Page View Product Specs

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CD4+ T cell infiltration into the CNS was measured by flow cytometry using tissue from mice with autoimmune neuroinflammatory disease (EAE).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse brain

Primary Incubation

1:100, 1 hour on ice

Blocking Agent

TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytoflex S Flow Cytometer (Beckman Coulter)

Results Summary

This antibody works very well to detect CD4+ T cells in mouse brain tissue.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High specificity, very bright dye

The Bad

Some emission overlap with PE-Cy7

The Bottom Line

This is the best CD4 antibody clone we've tested for flow cytometry and APC Fire 750 is a very bright dye.

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