Mouse EDTA Plasma

April 15, 2022

University of Washington
Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

RayBiotech

Product Name:

Mouse IL-1 beta IQELISA Kit

Catalog Number:

IQM-IL1b-1

We used EDTA plasma samples from mice with and without LPS and ATP treatments, and a sample from IL-1beta KO JAX mice (034447) as a negative control. We received positive signals regardless of the experimental condition. Recommend using the testing service provided by RayBiotech with an additional $5 service fee, as the kit is hard to conduct in-house.

Experimental Design and Results Summary

Application

ELISA

Starting Material

EDTA plasma from 1) untreated, 2) LPS+ATP treated, and 3) IL-1b KO mice

Tips

Recommend using the testing service provided by RayBiotech with an additional $5 service fee, as the kit is hard to conduct in-house.

Results Summary

The standard works well. We received ~300 pg/ml for control, 120 pg/ml for LPS+ATP, and ~280 pg/ml for IL-1b KO.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Standard works and can get signals for samples.

The Bad

The kit is hard to conduct in-house and provides non-specific signals.

The Bottom Line

According to the company, the kit works well as the standard works in their facility. Use the kit with caution, as the kit can provide a non-specific signal.

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