BD Biosciences
FITC Mouse Anti-Human PAC-1
340507
PAC-1
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Browse Similar Products: PAC1 Flow Cytometry Antibodies
PAC-1 recognizes the active conformation of GPIIb/IIIa, which mediates fibrinogen binding and platelet aggregation.
Flow Cytometry
Human whole blood collected in sodium citrate
30 min at room temperature
Staining buffer contains 0.5% BSA
N/A
Fluorescence
Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing FITC Mouse Anti-Human PAC-1 with or without 10 µg/mL CRP-XL (platelet agonist). Agonist-stimulated platelets show brighter staining for PAC-1, as expected.
Allows good staining of activated GPIIb/IIIa
FITC Mouse Anti-Human PAC-1 stains GPIIb/IIIa and allows good resolution of activated platelets.