Western Blot Collagen 4

April 14, 2022

Nanomedicine
Lazar Research Laboratories
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-Collagen IV antibody

Catalog Number:

ab6586

Clone Number:

N/A

Abcam antibody works well for Western blot, but results had several unspecific bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I got bands, but the quality is low.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band was detected.

The Bad

Too many unspecific bands.

The Bottom Line

The quality of the antibody is low, but it works.

Share your experience with other scientists. Write a Review! »

Join the discussion