Very Good DNA Cleanup Kit

April 18, 2022

BlueskyReddit
University of Oxford
Physiology Anatomy and genetics
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

NEB

Product Name:

Monarch® PCR & DNA Cleanup Kit (5 μg)

Catalog Number:

T1030L

Used to purify DNA after a ligation reaction, easy to remove RNA and other protein molecules such as enzymes attached to the DNA. Comes with all the buffers that are easy to buffer, although the wash buffer runs out when two wash steps are performed.

Experimental Design and Results Summary

Application

DNA purification

Starting Material

Impure DNA

Tips

Easy to use

Results Summary

Easy to use, however the wash buffer supplied is not enough if you want to perform double wash step

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Simple buffer, providede with all components and buffer that need to be diluted with ethanol.

The Bad

Wash buffer is limited and is not enough if multiple wash steps are performed.

The Bottom Line

It quick and easy kit. However, if performing multiple wash step you may need to order new kit as wash buffer which is not sold separately.

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