Santa Cruz Biotech
Anti-β-Actin Antibody (C4) Alexa Fluor® 680
sc-47778 AF680
N/A
Info:Supplier Page View Product Specs
Browse Similar Products: Beta-Actin Antibodies
We use this at 1:10,000 dilution in PBST with a licor western blot scanner and it works great - it's nice not to have to use a secondary antibody and the images have looked great. And it's in the 680 channel so you still have the stronger 800 channel for your weaker antibodies. No complaints, have reused it up to a month multiple times without any noticeable degradation (just stored at 4C)
Western Blot
K562, HeLa, U2OS
1:10,000 either overnight at 4C or RT for 1-2 hrs
Milk
LiCor
Easy loading control that always works
Cheap, reproducible, high signal
None
Would recommend