Good a-Smooth Muscle Antibody for Western Blot

Nanomedicine
Lazar Research Laboratories
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

α-Smooth Muscle Actin Antibody

Catalog Number:

14968

Clone Number:

N/A

Our research focuses on MRC-5 and the pSMAD pathway. I used this antibody for Western blot and got excellent results. I stimulated the cells with TGF-b and the pathway was activated; I was looking at one of fibrosis markers (a-SMA) and it was overexpressed once the cells were stimulated with TGF-b.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnightù

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Data looks really good.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High quality bands

The Bad

N/A

The Bottom Line

Really recommended.

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