Great BSA from Sigma

April 14, 2022

BlueskyReddit
Versiti Blood Research Institute
Translational Glycomics
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma Aldrich

Product Name:

Bovine Serum albumin Fraction V, heat shock, fatty acid free

Catalog Number:

03117057001

I used this product for doing my BSA gradient on Day 4 of Megakaryocyte culture and it gave excellent results.

Experimental Design and Results Summary

Application

For doing BSA gradient

Starting Material

BSA in PBS

Tips

Pipette out gradient very slowly

Results Summary

Gradient separation was excellent resulting in formation of a very nice pellet at the bottom of the Falcon tube.

DOI or PMID #

N/A

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Summary

The Good

Good gradient separation.

The Bad

There are no drawbacks of this product.

The Bottom Line

Used first 3% and then layered over 1.5% BSA gradient. Make sure to pipette out the gradient very slowly, so as to not mix the 2 gradients. Wait for 30-60 mins for a pellet to form.

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