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Alexa Fluor 647 anti-human CD42b Antibody
303924
HIP1
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CD42b (GPIba) is present on the platelet surface, where it mediates adhesion and aggregation.
Flow Cytometry
Human whole blood collected in sodium citrate
30 min at room temperature
Staining buffer contains 0.5% BSA
N/A
Fluorescence
Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing Alexa Fluor 647 anti-human CD42b. Lyse/fix buffer was added, and samples were acquired by flow cytometry. Alexa Fluor 647 anti-human CD42b provided bright staining and allowed the clear resolution of platelets from background.
Good staining of CD42b on platelets.
Alexa Fluor 647 anti-human CD42b gives bright staining for the clear resolution of platelets in whole blood.