Good CD61 Staining on Platelets

Hem/Onc
Boston Children's Hospital
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

R718 Mouse Anti-Human CD61

Catalog Number:

752050

Clone Number:

RUU-PL7F12

Info:Supplier Page View Product Specs

Browse Similar Products: CD61 Antibodies

CD61 is a β3 integrin that is present on the platelet surface, where it associates with integrinαIIb and mediates platelet-platelet aggregation. R718 Mouse Anti-Human CD61 provides good staining of CD61 on human platelets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood collected in sodium citrate

Primary Incubation

30 min at room temperature

Blocking Agent

Staining buffer contains 0.5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing R718 Mouse Anti-Human CD61. Lyse/fix buffer was added, and samples were acquired by flow cytometry. R718 Mouse Anti-Human CD61 allowed the clear resolution of platelets from background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining of CD61

The Bad

N/A

The Bottom Line

R718 Mouse Anti-Human CD61 provides good staining and allows the clear identification of platelets in whole blood.

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