Good CD36 Staining on Platelets

April 04, 2022

BlueskyReddit
Hem/Onc
Boston Children's Hospital
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

BV605 Mouse Anti-Human CD36

Catalog Number:

563518

Clone Number:

CB38

Info:Supplier Page View Product Specs

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CD36 is present on the platelet surface, where it modulates signaling via oxidized lipids and thrombospondin. BV605 Mouse Anti-Human CD36 allows good staining of CD36 on blood platelets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood collected in sodium citrate

Primary Incubation

30 min at room temperature

Blocking Agent

Staining buffer contains 0.5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing B605 Mouse Anti-Human CD36. Lyse/fix buffer was added, and samples were acquired by flow cytometry. This antibody allowed good staining of CD36.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining of CD36

The Bad

N/A

The Bottom Line

BV605 Mouse Anti-Human CD36 allows the clear resolution of CD36+ platelets from background.

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