Bright BV421 Rat Anti-Human TREML-1 Antibody

March 23, 2022

BlueskyReddit
Hem/Onc
Boston Children's Hospital
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

BV421 Rat Anti-Human TREML-1 (TLT-1)

Catalog Number:

748121

Clone Number:

268420

Info:Supplier Page View Product Specs

Browse Similar Products: TLT1 Antibodies

TREML-1 (TLT-1) is expressed on the platelet surface following activation. BV421 Rat Anti-Human TREML-1 (TLT-1) allows the discrimination of activated platelets from resting platelets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood collected in sodium citrate

Primary Incubation

30 min at room temperature

Blocking Agent

Staining buffer contains 0.5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing BV421 Rat Anti-Human TREML-1 (TLT-1) with or without 10 µg/mL CRP-XL (platelet agonist). Lyse/fix buffer was added, and samples were acquired by flow cytometry. BV421 Rat Anti-Human TREML-1 (TLT-1) allowed clear resolution of activated platelets.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright signal allows good resolution of activated platelets.

The Bad

N/A

The Bottom Line

BV421 Rat Anti-Human TREML-1 (TLT-1) allows the clear resolution of activated platelets.

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