Great Anti-R-Cadherin

March 11, 2022

BlueskyReddit
Cell Biology
Children's mercy hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Anti-R-Cadherin

Catalog Number:

51-9001934

Clone Number:

N/A

This antibody was chosen to stain human epithelial cells because it is a bright, commonly used clone for R-Cadherin that works well with other fluorophores in the staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human cancer tissue

Primary Incubation

1:250 dilution overnight incubation

Blocking Agent

5% GS

Secondary Incubation

Incubate secondary antibody in 5% gs in PBS for one hour with an orbital shaker at room temperature.

Tertiary Incubation

N/A

Detection

Confocal microscope.

Results Summary

R-cadherin staining was excellent. Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Results are very consistent.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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