BD Biosciences
Purified Mouse Anti-P-Cadherin
610227
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This antibody was chosen to stain for helper T cells since it is bright, works well with other fluorophores in the staining panel, and is a commonly-used clone for P-Cadherin. BD has a good reputation for making quality IF antibodies.
Immunofluorescence
Mouse lymph node
1:250 dilution overnight incubation
5% GS
Incubate secondary antibody in 5% gs in PBS for one hour with an orbital shaker at room temperature.
N/A
Cofocal
P-cadherin staining was excellent. Positive cells were fully resolved from negative.
Works well. Reproducible results.
Will continue to use this antibody.