Millipore Sigma
Anti-Choline Acetyltransferase Antibody
AB144P
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We use this antibody in our lab to mark cholinergic neurons and it works pretty well. In our experiments, we use a very standard IF protocol to detect these neutrons in PFA free floating slices.
Immunofluorescence
Mouse brain
Incubation was done with Ab diluted to 1:1,000 for 16h at 4ºC
BSA 4% in PBS
Incubation was done with 2nd Ab diluted to 1:1,000 for 1h at room temperature
None
Fluorescence (AF 488)
In all cases reactivity is quite good at 1:1,000 dilution. This protein is detected in only a few neurons from the mouse brainstem (green fluorescence).
Very good reactivity
I have experienced no issues with this product.
A very reliable antibody for studying cholinergic neurotransmission