Consistent Results in Alexa Fluor 488 Immunostaining

February 23, 2022

BlueskyReddit
Cardiovascular Biology
OMRF
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Jackson Immuno

Product Name:

Alexa Fluor® 488

Catalog Number:

705-546-147

Clone Number:

Our lab has routinely used this secondary antibody for immunostaining experiments to stain heart, liver, and lung tissue samples. Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For our experiment, this antibody staining is working very good.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse tissue liver and Heart

Primary Incubation

1:250 dilution overnight incubation

Blocking Agent

1% BSA prepared in 1X TBST

Secondary Incubation

1:250 dilution 1% BSA prepared in 1X TBST for 2hrs

Tertiary Incubation

None

Detection

Fluorescent microscope

Results Summary

This Secondary antibody is working very well and getting good and bright green signal in fluorescent microscope.

DOI or PMID #

NA

Additional Notes

NA

Image Gallery

Summary

The Good

Great

The Bad

None

The Bottom Line

Suitable secondary antibody and highly recommended to others.

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