Great Antibody for ULK1 Signaling Pathway

Department of Pediatrics
Children's mercy hospital
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Tech

Product Name:

Phospho-ULK1 (Ser757)

Catalog Number:

6888

Clone Number:

Info:Supplier Page View Product Specs

Browse Similar Products: ULK1 Antibodies

Our lab focus is on the study of pediatric leukemia in the context of ULK1 signaling. We are interested in developing new therapies and treatments for this disease. We are also interested in drug resistant leukemia.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymph node

Primary Incubation

Incubate primary antibody in 5% BSA in TBST overnight with an oribital shaker at 4*C

Blocking Agent

5% BSA in TBST

Secondary Incubation

Incubate secondary antibody in 5% BSA in TBST for one hour with an orbital shaker at room temperature

Tertiary Incubation

N/A

Detection

Amersham ECL Prime Western Blotting Detection Reagent

Results Summary

Cells were treated with several inhibitors overnight. Total p-ULK1 was used.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great signal strength

The Bad

N/A

The Bottom Line

This is a good antibody to use when you are interrogating ULK1 signaling in your cell of choice. It gives a nice clean signal quickly.

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