Straightforward Marker for Endothelial Cells

February 10, 2022

BlueskyReddit
Biotech
CNC
Research Scientist

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Quality of Results

Ease-of-Optimization

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Company:

Vector Labs

Product Name:

GSLI- DyLight 594 Labeled Griffonia Simplicifolia Lectin I (GSL I) isolectin B4

Catalog Number:

DL-1207

Clone Number:

Info:Supplier Page View Product Specs

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Normally used as a marker for endothelial cells of non-primates. We used to stain mouse brain endothelial cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse

Primary Incubation

20 ug/mL

Blocking Agent

10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2

Secondary Incubation

Already conjugated with 594

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

Staining of the membrane of endothelial cells

DOI or PMID #

Additional Notes

Related Categories

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Summary

The Good

Fast and easy protocol

The Bad

Staining should be done before adding any permeabilization agent, such as Triton X-100

The Bottom Line

Easy to implement than common antibodies to stain endothelial cells

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