BD Biosciences
PE-Cy™7 Mouse Anti-Human IgG
561298
G18-145
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining IgG in B cells (CD19+CD20+) in isolated PBMCs from a healthy donor (HD).
Flow Cytometry
Isolated PBMCs from HD peripheral blood
surface staining with IgG PE-Cy7 (0.1uL/50uL), 20 min at 4C
none
N/A
flow cytometer
We could stain IgG in B cells (CD19+CD20+) in isolated PBMCs from a healthy donor (HD).
Works well
It is a good option to stain human IgG.