Easy to Optimize ULK1 Antibody

January 28, 2022

BlueskyReddit
Department of Pediatrics
Children's mercy hospital
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb

Catalog Number:

#5869

Clone Number:

Info:Supplier Page View Product Specs

Browse Similar Products: ULK1 Antibodies

Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb detects endogenous levels of ULK1 only when phosphorylated at Ser555 of mouse ULK1 (equivalent to Ser556 of human ULK1). Bands of unknown origin are detected between 90 and 100 kDa.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymph node

Primary Incubation

1:1000 5% BSA 4deg O/N

Blocking Agent

5% GS

Secondary Incubation

1:10000 1% gs RT 1hr

Tertiary Incubation

N/A

Detection

ECL

Results Summary

A single band is obtained and it doesn't produce any non specific bands.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Results are very consistent.

The Bad

N/A

The Bottom Line

Highly recommend this antibody for those who wish to detect p-ulk1 expression.

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