Consistent Western Blot FIP200 Antibody

Pharmacology
KUMC
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

FIP200 (D10D11) Rabbit mAb

Catalog Number:

#12436

Clone Number:

Info:Supplier Page View Product Specs

Browse Similar Products: FIP200 Antibodies

FIP200 (FAK family kinase-interacting protein of 200 kDa) was identified in a two-hybrid screen with the tyrosine kinase Pyk2 and can inhibit Pyk2 kinase activity as well as related family members (1). FIP200 was later independently identified in a multi-drug resistance screen and named RB1CC1 (RB1-inducible coiled-coil 1) due to its induction by cytotoxic stress and RB1 expression regulation

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymph node

Primary Incubation

1:1000 5% BSA 4deg O/N

Blocking Agent

5% GS

Secondary Incubation

1:10000 1% gs RT 1hr

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We found time dependent increase of FIP200 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Results are very consistent.

The Bad

N/A

The Bottom Line

This antibody is very specific and the signal is strong.

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