Reliable Detection of Cleaved Caspase-3

Pharmacology
KUMC
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Tech

Product Name:

Cleaved Caspase-3 (Asp175) Antibody

Catalog Number:

9661

Clone Number:

The Cleaved Caspase-3 is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. Therefore this antibody is used to detect the activation of Cleaved Caspase-3 in cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymph node

Primary Incubation

1:1000 5% BSA 4deg O/N

Blocking Agent

5% GS

Secondary Incubation

1:10000 1% gs RT 1hr

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Two bands were detected as they represents the two different phosphorylated subunit of CAPS3. The bands were sharp and clear with no other no specific bands noted.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable, high quality, user friendly

The Bad

N/A

The Bottom Line

Highly recommended for those who wants to detect the level of CAPS3

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